Identifying Core Protein Complexes from Downscaled Tandem Affinity Purifications
نویسندگان
چکیده
منابع مشابه
Discovery of Protein Complexes with Core-Attachment Structures from Tandem Affinity Purification (TAP) Data
Many cellular functions involve protein complexes that are formed by multiple interacting proteins. Tandem Affinity Purification (TAP) is a popular experimental method for detecting such multi-protein interactions. However, current computational methods that predict protein complexes from TAP data require converting the co-complex relationships in TAP data into binary interactions. The resultin...
متن کاملTitle Discovery of protein complexes with core-attachment structures from tandem affinity purification (TAP) data
Many cellular functions involve protein complexes that are formed by multiple interacting proteins. Tandem Affinity Purification (TAP) is a popular experimental method for detecting such multi-protein interactions. However, current computational methods that predict protein complexes from TAP data require converting the co-complex relationships in TAP data into binary interactions. The resultin...
متن کاملIdentifying the topology of protein complexes from affinity purification assays
MOTIVATION Recent advances in high-throughput technologies have made it possible to investigate not only individual protein interactions, but also the association of these proteins in complexes. So far the focus has been on the prediction of complexes as sets of proteins from the experimental results. The modular substructure and the physical interactions within the protein complexes have been ...
متن کاملTargeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins
The molecular complexity of mammalian proteomes demands new methods for mapping the organization of multiprotein complexes. Here, we combine mouse genetics and proteomics to characterize synapse protein complexes and interaction networks. New tandem affinity purification (TAP) tags were fused to the carboxyl terminus of PSD-95 using gene targeting in mice. Homozygous mice showed no detectable a...
متن کاملIdentifying protein complexes from heterogeneous biological data.
With the increasing availability of diverse biological information for proteins, integration of heterogeneous data becomes more useful for many problems in proteomics, such as annotating protein functions, predicting novel protein-protein interactions and so on. In this paper, we present an integrative approach called InteHC (Integrative Hierarchical Clustering) to identify protein complexes fr...
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ژورنال
عنوان ژورنال: Journal of Integrated OMICS
سال: 2012
ISSN: 2182-0287
DOI: 10.5584/jiomics.v2i1.81